ATG16L1 Mouse Monoclonal Antibody [Clone ID: OTI5A2]

CAT#: CF809570

Carrier-free (BSA/glycerol-free) ATG16L1 mouse monoclonal antibody,clone OTI5A2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG16L1 (RC216728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG16L1 (1:500). Positive lysates LY410699 (100ug) and LC410699 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ATG16L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809570) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ATG16L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809570) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ATG16L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809570) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ATG16L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809570) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ATG16L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809570) (1:2000)

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Specifications

Product Data

• Clone Name: OTI5A2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-277 of human ATG16L1 (NP_110430) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.1 kDa
• Gene Name: Homo sapiens autophagy related 16 like 1 (ATG16L1), transcript variant 1, mRNA.
• Database Link:
  NP_110430
  Entrez Gene 77040 MouseEntrez Gene 363278 RatEntrez Gene 55054 Human
  UniProt ID Q676U5
• Background: The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
• Synonyms: APG16L; ATG16A; ATG16L; IBD10; WDR30