MEIS1 Mouse Monoclonal Antibody [Clone ID: OTI2A3]

CAT#: CF809619

Carrier-free (BSA/glycerol-free) MEIS1 mouse monoclonal antibody,clone OTI2A3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEIS1 (RC208135, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEIS1 (1:2000).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MEIS1 mouse monoclonal antibody.This figure shows negative staining. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MEIS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809619) (1:500)

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Specifications

Product Data

• Clone Name: OTI2A3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 176-390 of human MEIS1(NP_002389) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens Meis homeobox 1 (MEIS1), mRNA.
• Database Link:
  NP_002389
  Entrez Gene 4211 Human
  UniProt ID O00470
• Background: Homeobox genes, of which the most well-characterized category is represented by the HOX genes, play a crucial role in normal development. In addition, several homeoproteins are involved in neoplasia. This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. [provided by RefSeq, Jul 2008]
• Synonyms: MGC43380

Reference Data

• Protein Families: Transcription Factors