HIF 2 alpha (EPAS1) Mouse Monoclonal Antibody [Clone ID: OTI6G3]

CAT#: CF809645

Carrier-free (BSA/glycerol-free) EPAS1 mouse monoclonal antibody,clone OTI6G3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPAS1 (RC208604, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPAS1 (1:500). Positive lysates LY400555 (100ug) and LC400555 (20ug) can be purchased separately from OriGene.

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  HEK293T cells were transfected with the pCMV6-HIF1A (RC202461), pCMV6-EPAS1 (RC208604) and pCMV6-HIF3A (RC210255) (1:500).

Specifications

Product Data

• Clone Name: OTI6G3
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 584-870 of human EPAS1 (NP_001421) produced in E.coli
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.3 kDa
• Gene Name: Homo sapiens endothelial PAS domain protein 1 (EPAS1), mRNA.
• Database Link:
  NP_001421
  Entrez Gene 13819 MouseEntrez Gene 29452 RatEntrez Gene 2034 Human
  UniProt ID Q99814
• Background: This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
• Synonyms: bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma