NLRX1 Mouse Monoclonal Antibody [Clone ID: OTI4H8]

CAT#: CF809764

Carrier-free (BSA/glycerol-free) NLRX1 mouse monoclonal antibody,clone OTI4H8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLRX1 (RC211111, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLRX1 (1:2000). Positive lysates LY403011 (100ug) and LC403011 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-NLRX1 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NLRX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809764) (1:150)

Specifications

Product Data

• Clone Name: OTI4H8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 866-975 of human NLRX1 (NP_078894) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens NLR family member X1 (NLRX1), transcript variant 1, mRNA.
• Database Link:
  NP_078894
  Entrez Gene 270151 MouseEntrez Gene 315599 RatEntrez Gene 79671 Human
  UniProt ID Q86UT6
• Background: The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2013]
• Synonyms: CLR11.3; DLNB26; NOD5; NOD9; NOD26

Reference Data

• Protein Pathways: RIG-I-like receptor signaling pathway