PPP3CB Mouse Monoclonal Antibody [Clone ID: OTI2E4]

CAT#: CF809772

Carrier-free (BSA/glycerol-free) PPP3CB mouse monoclonal antibody,clone OTI2E4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP3CB (RC227763, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP3CB (1:2000). Positive lysates LY428054 (100ug) and LC428054 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 8 different cell lines by using anti-PPP3CB monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI2E4
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 23-96 of human PPP3CB (NP_001135826) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.9 kDa
• Gene Name: Homo sapiens protein phosphatase 3 catalytic subunit beta (PPP3CB), transcript variant 3, mRNA.
• Database Link:
  NP_001135826
  Entrez Gene 19056 MouseEntrez Gene 24675 RatEntrez Gene 5532 Human
  UniProt ID P16298
• Synonyms: CALNA2; CALNB; CNA2; PP2Bbeta

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway