PD L1 (CD274) Mouse Monoclonal Antibody [Clone ID: OTI13G7]

CAT#: CF809809

Carrier-free (BSA/glycerol-free) CD274 mouse monoclonal antibody,clone OTI13G7

Product Images

PD-L1 ELISA with 13G7 Capture (TA809809) and 9E12 Detection (TA808771) Antibodies. Substrate used: Recombinant Human PD-L1 (TP700201)

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  Immunocytochemistry staining of CD274 stable expression cells using anti-CD274 mouse monoclonal antibody (TA507087) (Left). The right is negative control (1:5000).

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  Flow cytometric Analysis of HCC78 cells, using anti-PDL1 antibody (TA809809), (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100).

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  Flow cytometric Analysis of stable expression CD274 cells using anti-CD274 antibody (TA809809) (Red) compared to a nonspecific negative control antibody (Blue). The left is 293T as negative control (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-PDL1 antibody (TA809809) (1:100).

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  Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right) using anti-PDL1 antibody (TA809809). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).

• Detection of PDL1 neutralizing antibody using MACS column. GFP+/PDL1+ 293T cells (co-transfected with PDL1 and GFP plasmid (RC213071, PS10010) were incubated with either PDL1 antibody TA809809 (red), non-specific antibody (green), isotype control (blue) or PBS (black) and then mixed with PD1+ 293T cells (RC210364) linked with magnetic-beads. The mixed cells were pulled down using MACS column (Miltenyi Biotec) and analysed by Flow Cytometry. GFP+/PDL1+ cells would not be collected if PD1/PDL1 interaction is neutralized by the tested antibody (1:50).

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Specifications

Product Data

• Clone Name: OTI13G7
• Applications: ELISA, FC, IF
• Recommended Dilution: IF 1:5000, FLOW 1:100
• Reactivities: Human, not react with Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-239 of human PD-L1/CD274(NP_054862) produced in HEK293 cells.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31 kDa
• Gene Name: Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.
• Database Link:
  NP_054862
  Entrez Gene 60533 MouseEntrez Gene 29126 Human
  UniProt ID Q9NZQ7
• Synonyms: B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)