SIRT1 Mouse Monoclonal Antibody [Clone ID: OTI5B2]

CAT#: CF809834

Carrier-free (BSA/glycerol-free) SIRT1 mouse monoclonal antibody,clone OTI5B2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT1 (RC218134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT1 (1:1000).

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-SIRT1 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and SIRT1-Knockout Hela cells (KO, Cat# LC810336) were separated by SDS-PAGE and immunoblotted with anti-SIRT1 monoclonal antibody TA809834. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SIRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809834) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SIRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809834) (1:2000)

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Specifications

Product Data

• Clone Name: OTI5B2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 552-676 of human SIRT1 (NP_036370) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81.5 kDa
• Gene Name: Homo sapiens sirtuin 1 (SIRT1), transcript variant 1, mRNA.
• Database Link:
  NP_036370
  Entrez Gene 93759 MouseEntrez Gene 309757 RatEntrez Gene 23411 Human
  UniProt ID Q96EB6
• Synonyms: SIR2; SIR2alpha; SIR2L1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors