CLCA1 Mouse Monoclonal Antibody [Clone ID: OTI8E7]

CAT#: CF809864

Carrier-free (BSA/glycerol-free) CLCA1 mouse monoclonal antibody,clone OTI8E7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLCA1 (RC218583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLCA1 (1:2000).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809864) (1:500)

Specifications

Product Data

• Clone Name: OTI8E7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 344-412 of human CLCA1(NP_001276) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens chloride channel accessory 1 (CLCA1), mRNA.
• Database Link:
  NP_001276
  Entrez Gene 1179 Human
  UniProt ID A8K7I4
• Background: This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine. [provided by RefSeq, Jul 2008]
• Synonyms: CACC; CaCC-1; CACC1; CLCRG1; GOB5; hCaCC-1; hCLCA1

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Olfactory transduction