Cryptochrome I (CRY1) Mouse Monoclonal Antibody [Clone ID: OTI2F5]

CAT#: CF809887

Carrier-free (BSA/glycerol-free) CRY1 mouse monoclonal antibody,clone OTI2F5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRY1 (RC207152, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRY1 (1:2000). Positive lysates LY418241 (100ug) and LC418241 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and CRY1-Knockout HeLa cells (KO, Cat#LC834622) were separated by SDS-PAGE and immunoblotted with anti-CRY1 monoclonal antibody TA809887 (1:200). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI2F5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 502-584 of human CRY1(NP_004066) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66.2 kDa
• Gene Name: Homo sapiens cryptochrome circadian regulator 1 (CRY1), mRNA.
• Database Link:
  NP_004066
  Entrez Gene 12952 MouseEntrez Gene 299691 RatEntrez Gene 1407 Human
  UniProt ID Q16526
• Background: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Jan 2014]
• Synonyms: PHLL1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Circadian rhythm - mammal