NeuN (RBFOX3) Mouse Monoclonal Antibody [Clone ID: OTI2E5]

CAT#: CF809952

Carrier-free (BSA/glycerol-free) RBFOX3 mouse monoclonal antibody,clone OTI2E5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBFOX3 (RC224826, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBFOX3 (1:2000). Positive lysates LY421190 (100ug) and LC421190 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-RBFOX3 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI2E5
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 29-97 of human RBFOX3(NP_001076044) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: Homo sapiens RNA binding fox-1 homolog 3 (RBFOX3), transcript variant 1, mRNA.
• Database Link:
  NP_001076044
  Entrez Gene 146713 Human
  UniProt ID A6NFN3
• Background: RNA-binding protein that regulates alternative splicing events. [UniProtKB/Swiss-Prot Function]
• Synonyms: FOX-3; FOX3; HRNBP3; NEUN