ALAS2 Mouse Monoclonal Antibody [Clone ID: OTI2D3]

CAT#: CF809959

Carrier-free (BSA/glycerol-free) ALAS2 mouse monoclonal antibody,clone OTI2D3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALAS2 (RC219079, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALAS2 (1:2000). Positive lysates LY424966 (100ug) and LC424966 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2D3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 139-287 of human ALAS2(NP_000023) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.5 kDa
• Gene Name: Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 1, mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_000023
  Entrez Gene 11656 MouseEntrez Gene 25748 RatEntrez Gene 212 Human
  UniProt ID P22557
• Background: The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
• Synonyms: ALAS-E; ALASE; ANH1; ASB; SIDBA1; XLDPP; XLEPP; XLSA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism