TEKT2 Mouse Monoclonal Antibody [Clone ID: OTI1E11]

CAT#: CF809970

Carrier-free (BSA/glycerol-free) TEKT2 mouse monoclonal antibody,clone OTI1E11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TEKT2 (RC207425, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TEKT2 (1:2000). Positive lysates LY415231 (100ug) and LC415231 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-TEKT2 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1E11
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-244 of human TEKT2(NP_055281) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.5 kDa
• Gene Name: Homo sapiens tektin 2 (TEKT2), mRNA.
• Database Link:
  NP_055281
  Entrez Gene 27285 Human
  UniProt ID Q9UIF3
• Background: This gene product belongs to the tektin family of proteins. Tektins comprise a family of filament-forming proteins that are coassembled with tubulins to form ciliary and flagellar microtubules. This gene is expressed in the testis and its protein is localized to the flagella of the sperms, indicating that it may play a role in spermatogenesis. [provided by RefSeq, Jul 2008]
• Synonyms: h-tektin-t; TEKTB1; TEKTIN-T