DDX19A Mouse Monoclonal Antibody [Clone ID: OTI3C12]

CAT#: CF809971

Carrier-free (BSA/glycerol-free) DDX19A mouse monoclonal antibody,clone OTI3C12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX19A (RC200751, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX19A (1:2000). Positive lysates LY413127 (100ug) and LC413127 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-DDX19A monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DDX19A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809971) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDX19A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809971) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DDX19A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809971) (1:500)

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Specifications

Product Data

• Clone Name: OTI3C12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DDX19A (NP_060802) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.8 kDa
• Gene Name: Homo sapiens DEAD-box helicase 19A (DDX19A), transcript variant 1, mRNA.
• Database Link:
  NP_060802
  Entrez Gene 13680 MouseEntrez Gene 292022 RatEntrez Gene 55308 Human
  UniProt ID Q9NUU7
• Background: ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19 functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins. [UniProtKB/Swiss-Prot Function]
• Synonyms: DDX19-DDX19L; DDX19L