AMDHD1 Mouse Monoclonal Antibody [Clone ID: OTI4C1]

CAT#: CF809977

Carrier-free (BSA/glycerol-free) AMDHD1 mouse monoclonal antibody,clone OTI4C1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMDHD1 (RC206763, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AMDHD1 (1:2000). Positive lysates LY407536 (100ug) and LC407536 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AMDHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809977) (1:1000)

Specifications

Product Data

• Clone Name: OTI4C1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AMDHD1 (NP_689648 ) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.6 kDa
• Gene Name: Homo sapiens amidohydrolase domain containing 1 (AMDHD1), mRNA.
• Database Link:
  NP_689648
  Entrez Gene 71761 MouseEntrez Gene 299735 RatEntrez Gene 144193 Human
  UniProt ID Q96NU7
• Synonyms: HMFT1272

Reference Data

• Protein Pathways: Histidine metabolism, Metabolic pathways