N myc interactor (NMI) Mouse Monoclonal Antibody [Clone ID: OTI2C1]

CAT#: CF809982

Carrier-free (BSA/glycerol-free) NMI mouse monoclonal antibody,clone OTI2C1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NMI (RC201543, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NMI (1:2000). Positive lysates LY417813 (100ug) and LC417813 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-NMI monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type A549 cells and NMI-Knockout A549 cells (KO, Cat# LC800044) were separated by SDS-PAGE and immunoblotted with anti-NMI monoclonal antibody TA809982 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI2C1
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-223 of human NMI(NP_004679) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.9 kDa
• Gene Name: Homo sapiens N-myc and STAT interactor (NMI), mRNA.
• Database Link:
  NP_004679
  Entrez Gene 9111 Human
  UniProt ID Q13287
• Background: NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]
• Synonyms: N-myc (and STAT) interactor; N-myc-interactor; N-myc and STAT interactor; N-myc interactor

Reference Data

• Protein Families: Druggable Genome, Transcription Factors