ESE1 (ELF3) Mouse Monoclonal Antibody [Clone ID: OTI6G6]

CAT#: CF809989

Carrier-free (BSA/glycerol-free) ELF3 mouse monoclonal antibody,clone OTI6G6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELF3 (RC225564, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELF3 (1:2000). Positive lysates LY426477 (100ug) and LC426477 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ELF3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ELF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809989) (1:250)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ELF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809989) (1:250)

Specifications

Product Data

• Clone Name: OTI6G6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:250
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 175-286 of human ELF3(NP_001107781) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.3 kDa
• Gene Name: Homo sapiens E74 like ETS transcription factor 3 (ELF3), transcript variant 2, mRNA.
• Database Link:
  NP_001107781
  Entrez Gene 13710 MouseEntrez Gene 304815 RatEntrez Gene 1999 Human
  UniProt ID P78545
• Synonyms: EPR-1; ERT; ESE-1; ESX

Reference Data

• Protein Families: Transcription Factors