RHOH Mouse Monoclonal Antibody [Clone ID: OTI9F4]

CAT#: CF809996

Carrier-free (BSA/glycerol-free) RHOH mouse monoclonal antibody,clone OTI9F4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RHOH (RC204324, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RHOH (1:2000). Positive lysates LY401372 (100ug) and LC401372 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI9F4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 97-190 of human RHOH(NP_004301) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.1 kDa
• Gene Name: Homo sapiens ras homolog family member H (RHOH), transcript variant 6, mRNA.
• Database Link:
  NP_004301
  Entrez Gene 74734 MouseEntrez Gene 305341 RatEntrez Gene 399 Human
  UniProt ID Q15669
• Background: The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2013]
• Synonyms: ARHH; TTF

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Leukocyte transendothelial migration