NSD3 (WHSC1L1) Mouse Monoclonal Antibody [Clone ID: OTI3B3]

CAT#: CF810070

Carrier-free (BSA/glycerol-free) WHSC1L1 mouse monoclonal antibody,clone OTI3B3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WHSC1L1 (RC212790, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WHSC1L1 (1:2000). Positive lysates LY413551 (100ug) and LC413551 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-WHSC1L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810070) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-WHSC1L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810070) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-WHSC1L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810070) (1:500)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WHSC1L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810070) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-WHSC1L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810070) (1:500)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-WHSC1L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810070) (1:500)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-WHSC1L1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810070) (1:500)

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Specifications

Product Data

• Clone Name: OTI3B3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 427-566 of human WHSC1L1(NP_060248) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.4 kDa
• Gene Name: Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3), transcript variant short, mRNA.
• Database Link:
  NP_060248
  Entrez Gene 54904 Human
  UniProt ID Q9BZ95
• Background: This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described. [provided by RefSeq, Jul 2008]
• Synonyms: NSD3; pp14328

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Lysine degradation