HACE1 Mouse Monoclonal Antibody [Clone ID: OTI6H8]

CAT#: CF810071

Carrier-free (BSA/glycerol-free) HACE1 mouse monoclonal antibody,clone OTI6H8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HACE1 (RC205602, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HACE1 (1:2000). Positive lysates LY412326 (100ug) and LC412326 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-HACE1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI6H8
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human HACE1 (NP_065822) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 102.2 kDa
• Gene Name: Homo sapiens HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 (HACE1), transcript variant 1, mRNA.
• Database Link:
  NP_065822
  Entrez Gene 209462 MouseEntrez Gene 361866 RatEntrez Gene 57531 Human
  UniProt ID Q8IYU2
• Synonyms: E3 ubiquitin protein ligase 1; HECT domain and ankyrin repeat containing

Reference Data

• Protein Families: Druggable Genome