SUGT1 Mouse Monoclonal Antibody [Clone ID: OTI7D2]

CAT#: CF810102

Carrier-free (BSA/glycerol-free) SUGT1 mouse monoclonal antibody,clone OTI7D2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUGT1 (RC201676, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUGT1 (1:2000). Positive lysates LY416475 (100ug) and LC416475 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-SUGT1 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and SUGT1-Knockout HeLa cells (KO, Cat# LC833528) were separated by SDS-PAGE and immunoblotted with anti-SUGT1 monoclonal antibody TA810102 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI7D2
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SUGT1(NP_006695) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.6 kDa
• Gene Name: Homo sapiens SGT1 homolog, MIS12 kinetochore complex assembly cochaperone (SUGT1), transcript variant 2, mRNA.
• Database Link:
  NP_006695
  Entrez Gene 67955 MouseEntrez Gene 290408 RatEntrez Gene 10910 Human
  UniProt ID Q9Y2Z0
• Synonyms: SGT1

Reference Data

• Protein Pathways: NOD-like receptor signaling pathway