MED28 Mouse Monoclonal Antibody [Clone ID: OTI6A3]

CAT#: CF810136

Carrier-free (BSA/glycerol-free) MED28 mouse monoclonal antibody,clone OTI6A3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED28 (RC204304, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED28 (1:2000). Positive lysates LY410842 (100ug) and LC410842 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6A3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MED28 (NP_079481) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.3 kDa
• Gene Name: Homo sapiens mediator complex subunit 28 (MED28), mRNA.
• Database Link:
  NP_079481
  Entrez Gene 66999 MouseEntrez Gene 80306 Human
  UniProt ID Q9H204
• Synonyms: 1500003D12Rik; EG1; magicin

Reference Data

• Protein Families: Transcription Factors