Endoglycan (PODXL2) Mouse Monoclonal Antibody [Clone ID: OTI3C2]

CAT#: CF810153

Carrier-free (BSA/glycerol-free) PODXL2 mouse monoclonal antibody,clone OTI3C2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PODXL2 (RC208583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PODXL2 (1:2000). Positive lysates LY414352 (100ug) and LC414352 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3C2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 366-486 of human PODXL2(NP_056535) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 64.9 kDa
• Gene Name: Homo sapiens podocalyxin like 2 (PODXL2), mRNA.
• Database Link:
  NP_056535
  Entrez Gene 50512 Human
  UniProt ID Q9NZ53
• Background: This gene is a member of the CD34 family of cell surface transmembrane proteins, which are characterized by an N-terminal extracellular mucin domain, globular and stalk domains, a single pass transmembrane region, and a charged cytoplasmic tail. The encoded protein is a ligand for vascular selectins. [provided by RefSeq, Oct 2012]
• Synonyms: EG; PODLX2

Reference Data

• Protein Families: Transmembrane