ZFYVE1 Mouse Monoclonal Antibody [Clone ID: OTI1A2]

CAT#: CF810160

Carrier-free (BSA/glycerol-free) ZFYVE1 mouse monoclonal antibody,clone OTI1A2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFYVE1 (RC211403, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFYVE1 (1:2000). Positive lysates LY405962 (100ug) and LC405962 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1A2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-308 of human ZFYVE1(NP_848535) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.1 kDa
• Gene Name: Homo sapiens zinc finger FYVE-type containing 1 (ZFYVE1), transcript variant 2, mRNA.
• Database Link:
  NP_848535
  Entrez Gene 217695 MouseEntrez Gene 299188 RatEntrez Gene 53349 Human
  UniProt ID Q9HBF4
• Background: The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate-containing membranes. This protein contains two zinc-binding FYVE domains in tandem and is reported to localize to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
• Synonyms: DFCP1; PPP1R172; SR3; TAFF1; ZNFN2A1