TLR8 Mouse Monoclonal Antibody [Clone ID: OTI4F3]

CAT#: CF810174

Carrier-free (BSA/glycerol-free) TLR8 mouse monoclonal antibody,clone OTI4F3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLR8 (RC221986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TLR8 (1:2000). Positive lysates LY408461 (100ug) and LC408461 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-TLR8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810174) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human spleen tissue within the normal limits using anti-TLR8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810174}) (1:2000).

Specifications

Product Data

• Clone Name: OTI4F3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 32-153 of human TLR8(NP_619542) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 119.6 kDa
• Gene Name: Homo sapiens toll like receptor 8 (TLR8), transcript variant 2, mRNA.
• Database Link:
  NP_619542
  Entrez Gene 51311 Human
  UniProt ID Q9NR97
• Synonyms: CD288

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway