CLEC10A Mouse Monoclonal Antibody [Clone ID: OTI2B10]

CAT#: CF810180

CLEC10A mouse monoclonal antibody, clone OTI2B10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLEC10A (RC207907, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLEC. Positive lysates LY405320 (100ug) and LC405320 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-CLEC10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810180) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CLEC10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810180) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CLEC10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810180) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-CLEC10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810180) (1:500)

Show all 5 images

Specifications

Product Data

• Clone Name: OTI2B10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 181-293 of human CLEC10A (NP_878910) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.3 kDa
• Gene Name: Homo sapiens C-type lectin domain containing 10A (CLEC10A), transcript variant 1, mRNA.
• Database Link:
  NP_878910
  Entrez Gene 10462 Human
  UniProt ID Q8IUN9
• Background: This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: CD301; CLECSF13; CLECSF14; HML; HML2; MGL

Reference Data

• Protein Families: Transmembrane