ATG4B Mouse Monoclonal Antibody [Clone ID: OTI1A3]

CAT#: CF810189

Carrier-free (BSA/glycerol-free) ATG4B mouse monoclonal antibody,clone OTI1A3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATG4B (RC216453, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATG4B (1:2000). Positive lysates LY403600 (100ug) and LC403600 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-ATG4B monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ATG4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810189) (1:2000)

Specifications

Product Data

• Clone Name: OTI1A3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATG4B (NP_847896) produced in HEK293T Cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.4 kDa
• Gene Name: Homo sapiens autophagy related 4B cysteine peptidase (ATG4B), transcript variant 2, mRNA.
• Database Link:
  NP_847896
  Entrez Gene 66615 MouseEntrez Gene 316640 RatEntrez Gene 23192 Human
  UniProt ID Q9Y4P1
• Background: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
• Synonyms: APG4B; AUTL1

Reference Data

• Protein Families: Protease
• Protein Pathways: Regulation of autophagy