NIRF (UHRF2) Mouse Monoclonal Antibody [Clone ID: OTI4E10]

CAT#: CF810202

Carrier-free (BSA/glycerol-free) UHRF2 mouse monoclonal antibody,clone OTI4E10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UHRF2 (RC219579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UHRF2 (1:2000). Positive lysates LY407258 (100ug) and LC407258 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-UHRF2 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI4E10
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-288 of human UHRF2(NP_690856) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 89.8 kDa
• Gene Name: Homo sapiens ubiquitin like with PHD and ring finger domains 2 (UHRF2), transcript variant 1, mRNA.
• Database Link:
  NP_690856
  Entrez Gene 109113 MouseEntrez Gene 309331 RatEntrez Gene 115426 Human
  UniProt ID Q96PU4
• Background: This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants some of which are non-protein coding. [provided by RefSeq, Feb 2012]
• Synonyms: NIRF; RNF107; TDRD23; URF2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors