CTLA4 Mouse Monoclonal Antibody [Clone ID: OTI8F1]

CAT#: CF810204

Carrier-free (BSA/glycerol-free) CTLA4 mouse monoclonal antibody,clone OTI8F1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTLA4 (RC213631, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTLA4 (1:500). Positive lysates LY421970 (100ug) and LC421970 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of human peripheral blood mononuclear cells (PBMCs) by using anti-CTLA4 monoclonal antibody. Left: Unstimulated PBMC. Right: PBMC stimulated with 10ug/ml Phytohemagglutinin for 3 days (1:100).

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  HEK293T cells transfected with either CTL4 (RC210150) plasmid (red) or empty vector control plasmid (blue).The cells were immunostained by anti-CTLA4 antibody and analyzed by flow cytometry (1:100).

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  Flow cytometric analysis of CTLA4 expression on stimulated human peripheral blood mononuclear cells (PBMC). Unstimulated PBMC, (a.left, b.blue), or 10ug/ml Phytohemagglutinin-stimulated (3 days) PBMC, (a.right, b.red), were stained with Anti-Human CTLA4 antibody (TA810204) (1:100).

Specifications

Product Data

• Clone Name: OTI8F1
• Applications: FC, WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CTLA4 (NP_001032720) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15 kDa
• Gene Name: Homo sapiens cytotoxic T-lymphocyte associated protein 4 (CTLA4), transcript variant 2, mRNA.
• Database Link:
  NP_001032720
  Entrez Gene 1493 Human
  UniProt ID P16410
• Background: This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008]
• Synonyms: ALPS5; CD; CD152; CELIAC3; CTLA-4; GRD4; GSE; IDDM12

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Autoimmune thyroid disease, Cell adhesion molecules (CAMs), T cell receptor signaling pathway