ZFP37 Mouse Monoclonal Antibody [Clone ID: OTI3E7]

CAT#: CF810209

Carrier-free (BSA/glycerol-free) ZFP37 mouse monoclonal antibody,clone OTI3E7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFP37 (RC220191, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFP37 (1:2000). Positive lysates LY418700 (100ug) and LC418700 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3E7
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZFP37(NP_003399) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71 kDa
• Gene Name: Homo sapiens ZFP37 zinc finger protein (ZFP37), transcript variant 3, mRNA.
• Database Link:
  NP_003399
  Entrez Gene 7539 Human
  UniProt ID Q9Y6Q3
• Background: This gene encodes a transcription factor that belongs to a large family of zinc finger proteins. A similar protein in mouse is thought to play a role in regulating the structures of the nucleolus and centromere in neurons. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
• Synonyms: zfp-37; ZNF906

Reference Data

• Protein Families: Transcription Factors