PPP4C Mouse Monoclonal Antibody [Clone ID: OTI2E4]

CAT#: CF810213

Carrier-free (BSA/glycerol-free) PPP4C mouse monoclonal antibody,clone OTI2E4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP4C (RC200538, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP4C (1:2000).

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  Western blot analysis of extracts (35ug) from 6 different cell lines by using anti-PPP4C monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PPP4C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810213) (1:2000)

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Specifications

Product Data

• Clone Name: OTI2E4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human PPP4C (NP_002711) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens protein phosphatase 4 catalytic subunit (PPP4C), transcript variant 2, mRNA.
• Database Link:
  NP_002711
  Entrez Gene 56420 MouseEntrez Gene 171366 RatEntrez Gene 5531 Human
  UniProt ID P60510
• Synonyms: PP-X; PP4; PP4C; PPH3; PPP4; PPX

Reference Data

• Protein Families: Druggable Genome, Phosphatase