NEGR1 Mouse Monoclonal Antibody [Clone ID: OTI3G8]

CAT#: CF810275

Carrier-free (BSA/glycerol-free) NEGR1 mouse monoclonal antibody,clone OTI3G8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEGR1 (RC216034, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEGR1 (1:2000).

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-NEGR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810275) (1:150).

Specifications

Product Data

• Clone Name: OTI3G8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 80-205 of human NEGR1 (NP_776169) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens neuronal growth regulator 1 (NEGR1), mRNA.
• Database Link:
  NP_776169
  Entrez Gene 59318 RatEntrez Gene 320840 MouseEntrez Gene 257194 Human
  UniProt ID Q7Z3B1
• Synonyms: DMML2433; IGLON4; KILON; Ntra

Reference Data

• Protein Pathways: Cell adhesion molecules (CAMs)