DHRS4L2 Mouse Monoclonal Antibody [Clone ID: OTI4A10]

CAT#: CF810277

Carrier-free (BSA/glycerol-free) DHRS4L2 mouse monoclonal antibody,clone OTI4A10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DHRS4L2 (RC215019, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DHRS4L2 (1:2000).

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  Western blot analysis of extracts (35ug) from Hepg2 cell line and mouse kidney tissue lysate by using anti-DHRS4L2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human placenta tissue within the normal limits using anti-DHRS4L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810277) (1:250)

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  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-DHRS4L2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810277) (1:250)

Specifications

Product Data

• Clone Name: OTI4A10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:250
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DHRS4L2 (NP_932349) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens dehydrogenase/reductase 4 like 2 (DHRS4L2), transcript variant 1, mRNA.
• Database Link:
  NP_932349
  Entrez Gene 317749 Human
• Synonyms: SDR25C3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Retinol metabolism