TANK Mouse Monoclonal Antibody [Clone ID: OTI1G2]

CAT#: CF810317

Carrier-free (BSA/glycerol-free) TANK mouse monoclonal antibody,clone OTI1G2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TANK (RC209759, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TANK. Positive lysates LY418162 (100ug) and LC418162 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from cell lines by using anti-TANK monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TANK-Knockout HeLa cells (KO, Cat# LC810199) were separated by SDS-PAGE and immunoblotted with anti-TANK monoclonal antibody TA810317 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TANK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810317) (1:500)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TANK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810317) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-TANK mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810317) (1:500)

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Specifications

Product Data

• Clone Name: OTI1G2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TANK (NP_004171) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.6 kDa
• Gene Name: Homo sapiens TRAF family member associated NFKB activator (TANK), transcript variant 1, mRNA.
• Database Link:
  NP_004171
  Entrez Gene 252961 RatEntrez Gene 10010 Human
  UniProt ID Q92844
• Background: The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
• Synonyms: I-TRAF; ITRAF; TRAF2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: RIG-I-like receptor signaling pathway