SMUG1 Mouse Monoclonal Antibody [Clone ID: OTI5G1]

CAT#: CF810345

Carrier-free (BSA/glycerol-free) SMUG1 mouse monoclonal antibody,clone OTI5G1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMUG1 (RC212141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMUG1 (1:2000). Positive lysates LY415367 (100ug) and LC415367 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5G1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human SMUG1 (NP_055126) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.7 kDa
• Gene Name: Homo sapiens single-strand-selective monofunctional uracil-DNA glycosylase 1 (SMUG1), transcript variant 1, mRNA.
• Database Link:
  NP_055126
  Entrez Gene 71726 MouseEntrez Gene 315344 RatEntrez Gene 23583 Human
  UniProt ID Q53HV7
• Synonyms: FDG; HMUDG; UNG3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair