TRAF5 Mouse Monoclonal Antibody [Clone ID: OTI7A3]

CAT#: CF810371

Carrier-free (BSA/glycerol-free) TRAF5 mouse monoclonal antibody,clone OTI7A3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAF5 (RC219243, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAF5 (1:500). Positive lysates LY417875 (100ug) and LC417875 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 6 different cell lines by using anti-TRAF5 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI7A3
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 334-557 of human TRAF5 (NP_004610) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 64.2 kDa
• Gene Name: Homo sapiens TNF receptor associated factor 5 (TRAF5), transcript variant 1, mRNA.
• Database Link:
  NP_004610
  Entrez Gene 7188 Human
  UniProt ID O00463
• Background: The scaffold protein encoded by this gene is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family and contains a meprin and TRAF homology (MATH) domain, a RING-type zinc finger, and two TRAF-type zinc fingers. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein is one of the components of a multiple protein complex which binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
• Synonyms: MGC:39780; RNF84

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Small cell lung cancer