MAP126 (SPAG5) Mouse Monoclonal Antibody [Clone ID: OTI3F10]

CAT#: CF810452

Carrier-free (BSA/glycerol-free) SPAG5 mouse monoclonal antibody,clone OTI3F10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPAG5 (RC201783, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPAG5 (1:2000). Positive lysates LY401941 (100ug) and LC401941 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-SPAG5 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and SPAG5-Knockout 293T cells (KO, Cat# LC812267) were separated by SDS-PAGE and immunoblotted with anti-SPAG5 monoclonal antibody TA810452, (1:200). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

Specifications

Product Data

• Clone Name: OTI3F10
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-297 of human SPAG5 (NP_006452) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 134.2 kDa
• Gene Name: Homo sapiens sperm associated antigen 5 (SPAG5), mRNA.
• Database Link:
  NP_006452
  Entrez Gene 10615 Human
  UniProt ID Q96R06
• Synonyms: DEEPEST; hMAP126; MAP126

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency