MED19 Mouse Monoclonal Antibody [Clone ID: OTI4C3]

CAT#: CF810475

Carrier-free (BSA/glycerol-free) MED19 mouse monoclonal antibody,clone OTI4C3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED19 (RC206806, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED19 (1:2000). Positive lysates LY407036 (100ug) and LC407036 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4C3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MED19 (NP_703151) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.3 kDa
• Gene Name: Homo sapiens mediator complex subunit 19 (MED19), transcript variant 2, mRNA.
• Database Link:
  NP_703151
  Entrez Gene 311165 RatEntrez Gene 381379 MouseEntrez Gene 219541 Human
  UniProt ID A0JLT2
• Background: MED19 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]). [supplied by OMIM, Oct 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037223.1, EL733643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS025082, ERS025095 [ECO:0000348] ##Evidence-Data-END##
• Synonyms: DT2P1G7; LCMR1; MED19AS

Reference Data

• Protein Families: Druggable Genome