NCOA7 Mouse Monoclonal Antibody [Clone ID: OTI1E12]

CAT#: CF810478

Carrier-free (BSA/glycerol-free) NCOA7 mouse monoclonal antibody,clone OTI1E12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NCOA7 (RC221673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCOA7 (1:2000). Positive lysates LY405613 (100ug) and LC405613 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-NCOA7 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1E12
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NCOA7 (NP_861447) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens nuclear receptor coactivator 7 (NCOA7), transcript variant 1, mRNA.
• Database Link:
  NP_861447
  Entrez Gene 135112 Human
  UniProt ID Q8NI08
• Synonyms: dJ187J11.3; ERAP140; ESNA1; Nbla00052; Nbla10993; NCOA7-AS; TLDC4

Reference Data

• Protein Families: Druggable Genome