AFAP (AFAP1) Mouse Monoclonal Antibody [Clone ID: OTI1E9]

CAT#: CF810491

Carrier-free (BSA/glycerol-free) AFAP1 mouse monoclonal antibody,clone OTI1E9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC207421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1 (1:5000). Positive lysates LY402869 (100ug) and LC402869 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-AFAP1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1E9
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AFAP1 (NP_067651) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant 1
• Database Link:
  NP_067651
  Entrez Gene 70292 MouseEntrez Gene 140935 RatEntrez Gene 60312 Human
  UniProt ID Q8N556
• Background: The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
• Synonyms: 110 kDa; actin filament-associated protein; actin filament associated protein 1; AFAP; AFAP-110; FLJ56849; OTTHUMP00000155170