CD79B Mouse Monoclonal Antibody [Clone ID: OTI8A12]

CAT#: CF810502

Carrier-free (BSA/glycerol-free) CD79B mouse monoclonal antibody,clone OTI8A12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD79B (RC200665, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD79B (1:2000). Positive lysates LY421857 (100ug) and LC421857 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-CD79B monoclonal antibody (1:500).

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  Western blot analysis of extracts (35ug) from 5 different cell lines and human spleen tissue lysate by using anti-CD79A monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD79B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810502) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD79B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810502) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD79B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810502) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD79B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810502) (1:2000)

• Flow cytometric Analysis of Jurkat cells, using anti-CD79B antibody (TA810502), (Red), compared to anti-CD3 antibody (TA807198), (Blue) (1:100).

• Flow cytometric Analysis of Ramos cells, using anti-CD79B antibody (TA810502), (Red), compared to anti-CD3 antibody (TA807198), (Blue) (1:100).

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Specifications

Product Data

• Clone Name: OTI8A12
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD79B (NP_001035022) produced in HEK293T.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens CD79b molecule (CD79B), transcript variant 3, mRNA.
• Database Link:
  NP_001035022
  Entrez Gene 974 Human
  UniProt ID P40259
• Background: The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
• Synonyms: AGM6; B29; IGB

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway