CDX1 Mouse Monoclonal Antibody [Clone ID: OTI2E8]

CAT#: CF810547

Carrier-free (BSA/glycerol-free) CDX1 mouse monoclonal antibody,clone OTI2E8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDX1 (RC220111, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDX1. Positive lysates LY419740 (100ug) and LC419740 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2E8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CDX1 (NP_001795) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28 kDa
• Gene Name: Homo sapiens caudal type homeobox 1 (CDX1), mRNA.
• Database Link:
  NP_001795
  Entrez Gene 12590 MouseEntrez Gene 1044 Human
  UniProt ID P47902
• Background: This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded DNA-binding protein regulates intestine-specific gene expression and enterocyte differentiation. It has been shown to induce expression of the intestinal alkaline phosphatase gene, and inhibit beta-catenin/T-cell factor transcriptional activity. [provided by RefSeq, Jul 2008]
• Synonyms: MGC116915

Reference Data

• Protein Families: Transcription Factors