RRM2 Mouse Monoclonal Antibody [Clone ID: OTI1F2]

CAT#: CF810548

Carrier-free (BSA/glycerol-free) RRM2 mouse monoclonal antibody,clone OTI1F2

Product Images

Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-RRM2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RRM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810548) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RRM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810548) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RRM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810548) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RRM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810548) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RRM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810548) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RRM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810548) (1:2000)

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Specifications

Product Data

• Clone Name: OTI1F2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RRM2 (NP_001025) produced in HEK293T.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens ribonucleotide reductase regulatory subunit M2 (RRM2), transcript variant 2, mRNA.
• Database Link:
  NP_001025
  Entrez Gene 20135 MouseEntrez Gene 362720 RatEntrez Gene 6241 Human
  UniProt ID P31350
• Background: This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Sep 2009]
• Synonyms: R2; RR2; RR2M

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways, p53 signaling pathway, Purine metabolism, Pyrimidine metabolism