HES5 Mouse Monoclonal Antibody [Clone ID: OTI6F7]

CAT#: CF810555

Carrier-free (BSA/glycerol-free) HES5 mouse monoclonal antibody,clone OTI6F7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES5 (RC215311, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES5 (1:500). Positive lysates LY423234 (100ug) and LC423234 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6F7
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HES5 (NP_001010926) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18 kDa
• Gene Name: Homo sapiens hes family bHLH transcription factor 5 (HES5), mRNA.
• Database Link:
  NP_001010926
  Entrez Gene 388585 Human
  UniProt ID Q5TA89
• Synonyms: bHLHb38

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Notch signaling pathway