FOXP3 Mouse Monoclonal Antibody [Clone ID: OTI6B10]

CAT#: CF810610

Carrier-free (BSA/glycerol-free) FOXP3 mouse monoclonal antibody,clone OTI6B10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXP3 (RC225614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXP3 (1:2000). Positive lysates LY426481 (100ug) and LC426481 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6B10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FOXP3 (NP_001107849) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens forkhead box P3 (FOXP3), transcript variant 2, mRNA.
• Database Link:
  NP_001107849
  Entrez Gene 20371 MouseEntrez Gene 317382 RatEntrez Gene 50943 Human
  UniProt ID Q9BZS1
• Background: The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
• Synonyms: AIID; DIETER; IPEX; JM2; PIDX; XPID

Reference Data

• Protein Families: Transcription Factors