RPB11 (POLR2J) Mouse Monoclonal Antibody [Clone ID: OTI1H4]

CAT#: CF810707

Carrier-free (BSA/glycerol-free) POLR2J mouse monoclonal antibody,clone OTI1H4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J (RC204819, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J (1:2000). Positive lysates LY401876 (100ug) and LC401876 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1H4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human POLR2J (NP_006225) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13.1 kDa
• Gene Name: Homo sapiens RNA polymerase II subunit J (POLR2J), mRNA.
• Database Link:
  NP_006225
  Entrez Gene 20022 MouseEntrez Gene 288588 RatEntrez Gene 5439 Human
  UniProt ID P52435
• Synonyms: hRPB14; POLR2J1; RPB11; RPB11A; RPB11m

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase