DDB2 Mouse Monoclonal Antibody [Clone ID: OTI2E12]

CAT#: CF810712

Carrier-free (BSA/glycerol-free) DDB2 mouse monoclonal antibody,clone OTI2E12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDB2 (RC200390, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDB2 (1:500). Positive lysates LY424922 (100ug) and LC424922 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2E12
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-250 of human DDB2 (NP_000098) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.7 kDa
• Gene Name: Homo sapiens damage specific DNA binding protein 2 (DDB2), transcript variant WT, mRNA.
• Database Link:
  NP_000098
  Entrez Gene 1643 Human
  UniProt ID Q92466
• Synonyms: DDBB; UV-DDB2; XPE

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair, p53 signaling pathway, Ubiquitin mediated proteolysis