IKB epsilon (NFKBIE) Mouse Monoclonal Antibody [Clone ID: OTI6H8]

CAT#: CF810713

Carrier-free (BSA/glycerol-free) NFKBIE mouse monoclonal antibody,clone OTI6H8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFKBIE (RC214683, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFKBIE (1:2000).

• 

  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-NFKBIE monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NFKBIE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810713) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NFKBIE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810713) (1:500)

Specifications

Product Data

• Clone Name: OTI6H8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NFKBIE (NP_004547) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens NFKB inhibitor epsilon (NFKBIE), mRNA.
• Database Link:
  NP_004547
  Entrez Gene 4794 Human
  UniProt ID O00221
• Synonyms: IKBE

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, B cell receptor signaling pathway, Neurotrophin signaling pathway, T cell receptor signaling pathway