Galectin 1 (LGALS1) Mouse Monoclonal Antibody [Clone ID: OTI1E2]

CAT#: CF810821

Carrier-free (BSA/glycerol-free) LGALS1 mouse monoclonal antibody,clone OTI1E2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS1 (RC204674, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS. Positive lysates LY400833 (100ug) and LC400833 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1E2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LGALS1 (NP_002296) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.5 kDa
• Gene Name: Homo sapiens galectin 1 (LGALS1), mRNA.
• Database Link:
  NP_002296
  Entrez Gene 16852 MouseEntrez Gene 56646 RatEntrez Gene 3956 Human
  UniProt ID P09382
• Background: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]
• Synonyms: GAL1; GBP

Reference Data

• Protein Families: Druggable Genome