Antizyme inhibitor 1 (AZIN1) Mouse Monoclonal Antibody [Clone ID: OTI3C3]

CAT#: CF810907

Carrier-free (BSA/glycerol-free) AZIN1 mouse monoclonal antibody,clone OTI3C3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AZIN1 (RC200022, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AZIN1. Positive lysates LY402474 (100ug) and LC402474 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-AZIN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810907) (1:2000)

Specifications

Product Data

• Clone Name: OTI3C3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 260-448 of human AZIN1 (NP_056962) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.4 kDa
• Gene Name: Homo sapiens antizyme inhibitor 1 (AZIN1), transcript variant 1, mRNA.
• Database Link:
  NP_056962
  Entrez Gene 58961 RatEntrez Gene 51582 Human
  UniProt ID O14977
• Synonyms: AZI; AZIA1; OAZI; OAZIN; ODC1L

Reference Data

• Protein Families: Druggable Genome