DNAJB5 Mouse Monoclonal Antibody [Clone ID: OTI6F11]

CAT#: CF810909

Carrier-free (BSA/glycerol-free) DNAJB5 mouse monoclonal antibody,clone OTI6F11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB5 (RC222132, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB. Positive lysates LY415870 (100ug) and LC415870 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 cell lines by using anti-DNAJB5 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DNAJB5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810909) (1:500)

Specifications

Product Data

• Clone Name: OTI6F11
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-261 of human DNAJB5 (NP_036398) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39 kDa
• Gene Name: Homo sapiens DnaJ heat shock protein family (Hsp40) member B5 (DNAJB5), transcript variant 3, mRNA.
• Database Link:
  NP_036398
  Entrez Gene 25822 Human
  UniProt ID O75953
• Background: DNAJB5 belongs to the evolutionarily conserved DNAJ/HSP40 protein family. For background information on the DNAJ family, see MIM 608375. [supplied by OMIM, Mar 2008]
• Synonyms: Hsc40